Table 1. Ceratodon purpureus EST intron primer sequences
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EST – intron position
|
5′ primer
|
3′ primer
|
5′ Tm
|
3′ Tm
|
Size*
|
|
|
|
|
|
|
Primer more than 25 nucleotides from the intron
|
|
|
|
|
AW098097 – 137
|
CCAATAGCAAAAGAATACAATCAAAGC
|
CGCGTTGTTCCTGGAATGG
|
62
|
64
|
318
|
AF233229 – 229
|
CCGAACTTGAACAGGACAGTTATGG
|
TGATCACGATTCCGACTGAGG
|
65
|
63
|
458
|
AF233229 – 433
|
GCTCCCAACTCAGGAACACCC
|
GGAGGCCTGGAGATGGTAACG
|
65
|
65
|
510
|
AW098318 – 384
|
AATCGTCACCGAATGAGAGGG
|
CAATGTTGGCATGCTCCC
|
63
|
61
|
467
|
AW098631 – 273
|
ATGTGTTGTGCTTTCCCAGG
|
TGGACAGTCACAACTCCTCTCCC
|
60
|
65
|
396
|
AW086794 – 139
|
TATCGGCGTGTGCAAGGG
|
GCTGGCTTCTCGAATGTGGG
|
64
|
65
|
342
|
AW086770 – 115
|
TCCGCGAGCTCTGAGTGG
|
AACAACTTCACCACATCTGCACG
|
63
|
64
|
308
|
AW086590 – 267
|
AACTGTGCACAAACAGCGGC
|
TGTACGACCATCCAGACTAAGAAGG
|
64
|
62
|
593
|
AW098359 – 150
|
GCGAAGTCCAAGAACCATACCG
|
AGCCTCGACCTCATCGGC
|
65
|
63
|
407
|
AW087018 – 146
|
CGACATTGCAGTACGAACTTTGC
|
CAAGTGGAGCCTGTCATCTTTGC
|
64
|
65
|
375
|
AW087018 – 260
|
CTGGTCATGGATCTGGTCGC
|
CAGTCCTCTCAGCATCCAGG
|
64
|
60
|
298
|
AW086837 – 165
|
CTTGGACGTGCATGGAGGC
|
GTGTTGAACCGCATGTTGTCG
|
65
|
64
|
316
|
AW086837 – 243
|
ATCAGCTTCGGACCCTTCACC
|
CTATGTGTGGGGCCAGCG
|
64
|
63
|
307
|
AW086675 – 86
|
CCGCATGTAGAAGAGCTACGCC
|
TCTCGTCGCAATGCAGGC
|
65
|
64
|
277
|
AW086645 – 117
|
ATGCTTGCAACGCTTGATGG
|
GTCAGGTTTCGGTGGTCAGTCC
|
64
|
65
|
466
|
AW098393 – 199
|
AAGGCTGAGGCTGAGGGC
|
AGTGCGGGAAGCCACACG
|
63
|
65
|
284
|
AW098679 – 454
|
CCTTGATGTGGTAGTAATCTGCG
|
CGAAATTGGTGGTGTGAGGG
|
60
|
63
|
438
|
AW098679 – 532
|
CATCGGGATTGAAAGCAAGAGC
|
ATTGGTGGTGGTCAAGAGGC
|
65
|
62
|
426
|
AW098515 – 203
|
CTGAAGAATTGGAACCAGTCCG
|
TTAAGCTTCGCGCAGTCCG
|
63
|
64
|
452
|
AW086579 – 194
|
ATCGGACGACTTCCTGCC
|
TTCAGGAATACGGTCGGCG
|
61
|
64
|
223
|
AW086579 – 332
|
TGCAACTCCCAAAGCTGCC
|
TGTTTTGGCACGAGACATGG
|
64
|
63
|
412
|
AW086736 – 262
|
GAGGTCCGAAGAGAGCCACC
|
TCTTGCATGTGGCAGACACG
|
63
|
64
|
404
|
AW097953 – 298
|
GCCCCTTCAAGAGACTTTGGC
|
GGCAAGAACATCTTGTCTTTGATGC
|
64
|
65
|
354
|
AW098418 – 107
|
GAAGAAGGAGACGCCCGC
|
AGACGGTCGGACAACACGG
|
63
|
64
|
320
|
AW098974 – 394
|
TTGGAAGCCAATTTCTCCTTGG
|
CAGGCACAGGAAGACTGTGAACC
|
64
|
65
|
510
|
AW086877 – 265
|
TTGATCCTCGGCTAGTTGCTCC
|
TCCAGTGCTTCCAGCAGCC
|
64
|
65
|
511
|
AW086877 – 378
|
ATGGAGATGCGGCTGCTGG
|
ACCAAGTGAATGCGGTCATATCC
|
67
|
64
|
339
|
AW086549 – 119
|
ACAAGGAAAGACGCAATTATTTATAGG
|
TGTGTCCTCCGGCTCACG
|
61
|
64
|
394
|
AW086549 – 238
|
AGAGCACAGGCATGACGGC
|
GCAGAATTGTATGCGCGACG
|
64
|
64
|
323
|
AW086868 – 74
|
CGATGTGGTTTACAACGCGTCC
|
ACCAGGTACGGAAGGGGGC
|
66
|
65
|
377
|
AW086868 – 247
|
TCGATGCTGCCCCCTTCC
|
CAGAACGGCCGCATTGG
|
66
|
64
|
392
|
AW086594 – 170
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TGCTCCAACAAGTCACCAATGC
|
TTCCCATTGACTTGCGCC
|
65
|
63
|
337
|
AW086594 – 327
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GCTTGGTCAACCTCCTGCG
|
TGAGAGTTGCCAAGGTCACTGG
|
64
|
64
|
479
|
AW098340 – 132
|
GCCCAGCAACAGCAAGGG
|
CACCACCCTGGCCAACTCC
|
65
|
66
|
441
|
AW098700 – 545
|
CCACTTCTGCCATGGTGGG
|
GCTTCGGTGTGGTTCTGTGG
|
64
|
64
|
350
|
AW086759 – 94
|
CCGTAGCTTCTGCAATATGTTTGAGG
|
TGGCAAGGCGACCAAAGC
|
65
|
65
|
486
|
AW098624 – 132
|
GACAAGGCCAGTTTGTACGCC
|
TGGTCTTAGCCTTATTGTGAAAGACG
|
64
|
63
|
269
|
AW098020 – 216
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TCCGTTCAATTCAGCTCGG
|
TGACGCAGCTTAGCGGGC
|
62
|
65
|
262
|
AW098462 – 157
|
TGACGATAGAAGGTGTGCGCC
|
AACAGGAAGGCCAGCCTATGG
|
65
|
64
|
576
|
AW086686 – 370
|
CACTCCCTTTTTGAGATCTTCAGGG
|
ATGGGGTGCAGATTGGGG
|
65
|
64
|
396
|
AW098568 – 216
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ACCACATCCATTTCGGACGC
|
TTCCCAGCTCGACAACATGG
|
65
|
64
|
327
|
AW086975 – 261
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ACTTATTTCGCCAGAGGATCTGC
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TCACTTTGACTCACAGACTGAATGACC
|
63
|
65
|
391
|
AW086975 – 331
|
TTGCGCAAGTTTGACAGTGG
|
GCAGGAGCCATTCCCAAGG
|
63
|
65
|
362
|
AW098234 – 164
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TGGAGCTTGCTCACATCGC
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CAGCAATACCCACTGCACGG
|
63
|
64
|
396
|
AW098407 – 97
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GGCGAAGGCTGTGATGGG
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TTTTTGCAAATGCAACCTGGG
|
65
|
65
|
392
|
AW086555 – 375
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GCCATAGTCGCATCAAAATTGG
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GGTTGTGGTCAGCAGCCG
|
63
|
63
|
438
|
AW097994 – 187
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ACGAAGCTGGAGGCTGTGG
|
CAATGTCTATGGAACCAATCTTTGC
|
63
|
62
|
396
|
AW086517 – 380
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TTGGTCTTCCTCCGCAGC
|
TCCGTGGATGATGATCTGGC
|
62
|
64
|
473
|
AW086517 – 457
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CATGTCTTGCTTCAAGGATGC
|
TCTGCATTGGAGGCAGAATCCG
|
60
|
68
|
267
|
AW098225 – 85
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AGGCCAGAGTTGCTGAACCG
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ACTCACCCGCCAGCAAGG
|
65
|
64
|
531
|
AW098839 – 86
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TGACTAGATGCTTTTATTGCTGAAAGG
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ATCACATCGAACGGGCAGC
|
63
|
64
|
493
|
AW098839 – 177
|
CAACCTGGTCCAGATCACGC
|
TACGGTGGCCGCAAGAGC
|
64
|
65
|
428
|
AW086694 – 137
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TCTTCATGACCTTAGCCACAGCC
|
AGATGATCTTGATGCGAGGG
|
64
|
60
|
304
|
AW086845 – 149
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AGGGGAAAGGCACCAGGG
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AGTTCACGGTTCCGGTGAGG
|
64
|
64
|
679
|
AW086858 – 91
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CATCAAGGGAGTGGGGCG
|
TCAGCTTCATGTCCAAGGCG
|
64
|
64
|
451
|
AW086858 – 280
|
TGGTGATCGTTGCCAACCC
|
CACCGACGGTTCTTCCACG
|
65
|
64
|
406
|
BI894288 – 61
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TGGAAGAAGCCTTTTGCTGG
|
TTCAAACAACCATCGTTGGGG
|
62
|
65
|
285
|
BI894288 – 188
|
GGCTAAGCAGCCCAACTCTGC
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TCTCCCACGGCATGTCCC
|
65
|
65
|
353
|
AW087023 – 114
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GGATCCACAAGGCCAAGTTGC
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CATTTCGACGAACGGTGGC
|
66
|
64
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433
|
AW087023 – 239
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AGCCATAAACGCAATTCGGG
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TGACTGAACCCGTCCGGC
|
64
|
65
|
399
|
AW097931 – 294
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AGAAGTTCCAAGTCGGGTGGC
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TCCGTTGGCGTTCTTCAGC
|
64
|
64
|
500
|
AW098613 – 99
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CTTTGGCTTGAGAAACAAGGGG
|
GCCGGCGATACAAATGAAACC
|
64
|
65
|
251
|
AW086659 – 247
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TTCCAGGTGGGTGGGAGC
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AGGGTTTGACCCCGTCGC
|
64
|
65
|
494
|
AW086999 – 142
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AGCTATTAGGGCGAGTGAAAGCG
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GGCTGGAGTGAATCATTTTGGG
|
65
|
64
|
368
|
AW086548 – 172
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AAATTCGGCAAATAAGAGGGG
|
CCGGGCAAATCGTTCAGG
|
61
|
64
|
428
|
AW098157 – 126
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CCTGCGAGGAAGATGACGC
|
AAGTTTCCACATTCAGAAGAGCCG
|
64
|
64
|
514
|
AJ250735 – 923
|
TCAGCCAGTTTGATGGGC
|
GGTTTCCATCCGGGGAGC
|
60
|
65
|
723
|
AJ250735 – 1460
|
TCAGGCCCGAGTTGACCC
|
AAGTGCCCGAAGCCATGC
|
64
|
64
|
747
|
AW097975 – 117
|
GAAGGGAAAGCCTGATTTGCG
|
GATGGGTCCGGTGATGGC
|
65
|
64
|
564
|
AW098252 – 97
|
CAAGGAGGTGGCGACTGC
|
CCGGCGAACTGCAAAACC
|
63
|
64
|
624
|
AW086519 – 461
|
CAGCGAAAATCAGACGCTGC
|
CCGCAAGGACCATCACCC
|
64
|
64
|
1025
|
AW098432 – 169
|
ATGAGTCGGCCGCATGG
|
TGCAGAGCCTTACACGACGC
|
64
|
64
|
759
|
AW087005 – 110
|
GCTATGTGGTGGCGTCCG
|
GAACAGCAAAATATTGCCTCTCGG
|
63
|
65
|
385
|
AW098158 – 174
|
TCCCTTGCCCTTCTTGTCTCC
|
TGTGCTGAGGTTGCAGTGCC
|
64
|
65
|
216
|
AW098158 – 249
|
TGCTGTGTCACGTGCTTGC
|
GGAAGCAGTCAGGATACGGAGG
|
63
|
64
|
443
|
AW098158 – 317
|
CGGGCTTGGTCTGTCCTCC
|
TCTTCTGCCCTGGGAAGGC
|
65
|
65
|
471
|
AW098372 – 71
|
TCCATTTGATCGCTGTTCCG
|
GAACTCAGGCAATCAAAGCAAGC
|
64
|
64
|
257
|
AW097984 – 288
|
GTGGGCTGAAGCGCAAGC
|
TGATCATGTTGCGTAGGTGGG
|
65
|
64
|
237
|
AW098512 – 413
|
TCGCAACTCAGCCCTCACG
|
TTGTTCCCGCAAGTGGGC
|
66
|
65
|
319
|
AW098442 – 86
|
AGCTCTGCACTGGGCTGG
|
TGGAACCGTGAAAGCCCG
|
62
|
65
|
279
|
AW098442 – 198
|
GGATAAGCAGACGCGCCG
|
CGAAAATGCAACCCCAGGC
|
65
|
65
|
271
|
AW098442 – 292
|
CCCAAAGTTCTTCTTGGGGC
|
GAAAGATGTGAAGAAGCTGTTGAAGC
|
63
|
63
|
252
|
AW098349 – 75
|
AGATGATGCTGAGATGGAAGCG
|
TCAAGAGAGGCACCCTCGG
|
63
|
63
|
400
|
AW098572 – 84
|
TGGGCTCTTGATGTTCTGGG
|
ACCGATCCTCAGCCTGCC
|
63
|
63
|
381
|
AW098572 – 303
|
CCACAAATGGATCGGCAGG
|
TTGCCTTCAAGCACAGCAGC
|
64
|
65
|
316
|
AW086786 – 104
|
ACGTGCTTGTTGCAAGGGC
|
AACAGCACGTGTCGGTCAGC
|
64
|
64
|
291
|
AW086989 – 141
|
AAGTGGCGCGGTTTGAGG
|
GTTTGACAGGAACGGGCAGG
|
64
|
65
|
759
|
AW098304 – 85
|
ATGGAAGCATGTGGTTTTGGG
|
CGACGCATGTCCTTCGGC
|
64
|
66
|
560
|
AW086668 – 76
|
CAACAGGCCTCTAAATCTTGGCG
|
TGGAGATTCTCACAGCCCCC
|
66
|
64
|
307
|
AW098058 – 71
|
CCAAGCGGGGCTTGTATGC
|
GTCCAGCTTGCGTTCGGG
|
66
|
65
|
414
|
AW098449 – 310
|
CTGCCAAGACCATTTTGAGTGG
|
AAAAAGTCACGACTAATGCTGTGC
|
64
|
61
|
369
|
AW097976 – 116
|
CATGTGCTGGTCTCACATTCCC
|
GCATTTTGTCTCAAGCCTTCG
|
65
|
62
|
485
|
AW098621 – 172
|
TCGTGGTTGAGTTGAACGGG
|
TCCCAAAACCTTGCCTCCG
|
64
|
65
|
407
|
AW097924 – 87
|
GCGTGCGTATGTTGTGATTAAGG
|
GCTCTCTGCTACAGCAGCCTCG
|
63
|
66
|
347
|
AW097924 – 222
|
CATGTTGCGCGAATCAGAGG
|
AACACAGAGTCTTGAATCTCCAGGG
|
65
|
64
|
323
|
AW086546 – 344
|
TCCTTCTCGGCAGCCTCG
|
CAACCGTCTTAACACCTACAGGGC
|
65
|
65
|
504
|
AW086789 – 334
|
GACCCCCAGAGCATCTTCG
|
CCTTGCGCTCCTTGATCTGC
|
63
|
65
|
358
|
AW098343 – 365
|
CCAACGACGCTTCTTCGC
|
GAGGACGGGATTTGTTCATGC
|
63
|
63
|
674
|
AW098256 – 171
|
TGATGACGAGCTGCTCAGAGG
|
GCCTTCTCAGCTTTGGCTGC
|
64
|
64
|
310
|
AW098256 – 290
|
AAGTGGCCGTGGAGATACAAGC
|
GCTGCTCTCTCCTTCTCACGC
|
64
|
64
|
393
|
AW097983 – 255
|
TGCAAGCCCAGCTCTCTGC
|
TCAAATTCCATTTGCTCGTCC
|
65
|
62
|
455
|
AW097983 – 358
|
GTGGCAATATTAAAGAGGCACCG
|
GGAATACATGCTGCTCCTTTCTCC
|
63
|
64
|
307
|
AW087043 – 267
|
CCCTTGCCCTTCTTCTTCTGG
|
TTCTCACTTGGAGGATCAGTTTGC
|
64
|
63
|
764
|
AW086539 – 362
|
GTTCCTGCAGCGCAGTCG
|
ACTTGCGCCAAGGAGAATGG
|
64
|
64
|
425
|
AW098322 – 137
|
CTTGGGAAGACGTGGCCC
|
AAGCGCTTCACCCAGGC
|
63
|
62
|
339
|
AW098322 – 275
|
CGTCATTGATGCTGAGGCG
|
CAAGCCGACGCCAGTTGC
|
63
|
66
|
398
|
AW097946 – 284
|
CGACGCCGACATTCATGC
|
AGGTCTGCATTCAGCAGATTACCC
|
64
|
64
|
370
|
AW098391 – 123
|
AGCCGAGGATTTAGGGCAGG
|
AGACCGGTGCCTCATCCG
|
64
|
64
|
287
|
AW098391 – 191
|
AGGTGCTTGGACTGCTCAGG
|
GGAATGCCAAAGGCGTCG
|
62
|
64
|
297
|
AW097948 – 171
|
GGCCCAAATCACCTACGCC
|
GTTCTTCAGATGACGACGAGCC
|
64
|
63
|
408
|
AW097948 – 251
|
TGGTCTTCTTTTGGCTCGTCG
|
CGTCCAACAGAGCCCTAAATGG
|
64
|
65
|
244
|
AW098786 – 108
|
GCACCGGTGGAAAGACCG
|
ACGTATCACGAGGGCCACC
|
65
|
63
|
349
|
AW098786 – 315
|
ATGGGAAGACTTGCGCGG
|
TTTGTGACCCATTCGCCC
|
64
|
62
|
324
|
AW098048 – 269
|
TCCCAGGTATCATTGCCCG
|
AATGAAAGTGGCTGCTATCCAAGC
|
64
|
64
|
606
|
AW098048 – 348
|
ACAGATGTGGCTTGGATAGCAGC
|
CCGATGTAAGTGTCTCTGCTGATGG
|
64
|
66
|
333
|
AW098804 – 195
|
GGCGGAGACAGGCACATACC
|
GAGAATTCAGGCCGCTCCG
|
65
|
65
|
405
|
AW086917 – 156
|
GAATGGCCTCTTCCGGGC
|
TGCAATCTGCACCGACTTGG
|
65
|
65
|
333
|
AW086712 – 250
|
TTACTGCCTTGCTGGGGTCC
|
AACCAGCCCGCTAAGCTGC
|
64
|
64
|
378
|
AW087002 – 282
|
TTATCGCCAGAGGACAGCG
|
GCCCATATTTGACAAGGCCG
|
62
|
64
|
402
|
AW086924 – 329
|
GCAGCACCTTGACGTCGC
|
AGCTTCAAGGCCTGAGAAATGC
|
64
|
64
|
600
|
AW098761 – 71
|
CATGGCGCAGACCAATACG
|
AAGTATGGGATCAAAGAGTTGTAGAGC
|
63
|
61
|
294
|
AW086619 – 136
|
CACACCTGCCTGTGGATTTGG
|
CAAGCTCGACAACCCGGC
|
65
|
65
|
602
|
AW087029 – 297
|
CATGGGGTTCTGAGGCCG
|
GGTCGGGATGAAATGCAAGC
|
64
|
64
|
486
|
AW086641 – 183
|
GGTTGCCTCCCTCAATCGC
|
GAAGGCTGTCGGCATCTGG
|
65
|
64
|
248
|
AW086641 – 266
|
AAGGTGTAAGCACCTCCGGC
|
GAAAGCCGGAATCGTCGG
|
63
|
63
|
501
|
AW086618 – 269
|
TGCCTCCTTCGCCTGATCC
|
AGACATCGGAAAAGAAGTCGAACG
|
66
|
64
|
513
|
AW098004 – 106
|
GGATTGGGCGAAAGAAGCG
|
AGAGAAGCACAACAGGGCCG
|
65
|
65
|
363
|
AW098004 – 215
|
CCATGGCTCGGAAGGAGG
|
CCTCCGTAAGGCTGACTGTCC
|
64
|
63
|
373
|
AW098782 – 68
|
GCTTGCTCCTGCTTTGACTGC
|
CTGAAGGGCTCCCAAATAGCC
|
64
|
64
|
422
|
AW098479 – 502
|
CTGGACAGCCAGTCAAGGC
|
CCGATGCAATGAATGCCG
|
62
|
64
|
281
|
AW086833 – 142
|
AAGGATCCGCGACAAGTACCC
|
TTGATGCGCTTCCTTATGACG
|
64
|
63
|
298
|
AW098949 – 71
|
GATTTCAAGACGCATCAGTTGCC
|
CGAAGATGTCGCCCCTGG
|
65
|
65
|
466
|
AW098949 – 266
|
TGGCGCTATTACCAGGGGC
|
CTGTCCAGGCATGGTGGG
|
65
|
63
|
406
|
AW098098 – 311
|
CCCCTGATCTTCTTGGCACG
|
GACCCCCAGAGCATCTTCG
|
65
|
63
|
349
|
AW086738 – 110
|
CATGCAGAACGAGGAGGGC
|
CCTGAGCGCGAACGAAGC
|
64
|
65
|
528
|
AW086979 – 128
|
TACACATTGCAGCGCATGG
|
CCATAACAATGAAGGCGCACG
|
62
|
65
|
342
|
AW086878 – 120
|
TCTACCCCGCCGATGACG
|
GAGAAGGCCAGACTCCAACTGC
|
65
|
64
|
654
|
AW087022 – 123
|
CGCCTTCACAGGCACAGG
|
CTTGGAAGCCAATTTCTCCTTGG
|
64
|
65
|
502
|
AW086647 – 346
|
AGATGATGGGGGTGGTGTAAGC
|
GCAACCCCATTGAGAAAGCG
|
64
|
65
|
395
|
AW098140 – 138
|
CCTAGTCACATGCCATGACCACC
|
CGCTCAGGGAGATACTGACAGC
|
65
|
63
|
412
|
AW097944 – 86
|
AAGGCCGTGTCCAGCTCC
|
TCGGGGCTAACAATGCACC
|
63
|
64
|
236
|
AW097944 – 242
|
CGTAACGGTGAGCGCAGG
|
TTGCCTGTGGCAGACACG
|
63
|
63
|
538
|
AW087030 – 122
|
ACTGTTGGAGGATGGTCGTCG
|
CCTCCATCAACAGCCGAGG
|
64
|
64
|
536
|
AW086635 – 160
|
TTGACTGCGAACTTCCTCATGG
|
GGCTTTCAATCGCCCAGG
|
64
|
63
|
380
|
AW098279 – 292
|
TTTCGCGACATGGATGGG
|
AAGTCTCTACCTCTATTGCATCAAGCC
|
64
|
63
|
260
|
AW086909 – 334
|
ACGTCCTCTGCGTCCTCCC
|
TCCGCTCGTGTCACTGGG
|
65
|
64
|
412
|
AW086827 – 136
|
CTCGGATCCGCGTGTTGC
|
CGTACGCGGACTCTGGTGG
|
66
|
65
|
790
|
AW086674 – 69
|
CTGCTCTTGCCAGTCTTGAACC
|
GAACACTGTGACTGCTGAGAAGTTGG
|
63
|
65
|
352
|
AW098248 – 320
|
TTGGACGCCATCTTCGGC
|
CTGCCTCTCCTGTGACAAAAGC
|
65
|
63
|
571
|
AW098328 – 149
|
TCAAGATGGAGGTGGGAATCG
|
GCCCCGTCCATGATTTCG
|
64
|
64
|
335
|
AW097937 – 210
|
TCAAATGCGATGAATGTCGC
|
CTGGACAGCCAGTCAAGGC
|
63
|
62
|
291
|
AW086669 – 414
|
AACGACCAGCGTAGGTGCC
|
GCTGAGAAGGGTGAAGATGCG
|
63
|
64
|
290
|
AW098185 – 232
|
TATTGCGACTGCCCCACG
|
GGGGTTATGCTACGGCACG
|
64
|
63
|
166
|
AW087017 – 243
|
CGATCCTGAAACAGGCCACC
|
GAACTTTGCCTCAAACTTTCCAGC
|
65
|
64
|
445
|
AW087017 – 331
|
TTGGTGGTGGTCAAGAGGC
|
AAAGCAAGAGCATTGATAGGTCCG
|
62
|
64
|
382
|
AW086830 – 254
|
TTTGGGTGGCCTTCTTCAGC
|
GGGAGACACCCAACCACTTCG
|
64
|
66
|
533
|
AW086566 – 259
|
TGGGTAGCTCCAATACCGGG
|
TTCCAGCGTGGGTTGAAGC
|
64
|
64
|
431
|
AW086566 – 452
|
CGATGATCATGTTGCGGAGG
|
TCCGGGGAGTGGATCTCG
|
64
|
64
|
606
|
AW098560 – 101
|
ATCCGCAAGTGCAAGCCC
|
TCATCCGTGGTGATTTTCGG
|
64
|
64
|
420
|
AW098281 – 150
|
GTACTTCGAAGACAATGCGGG
|
CATTCGCAGCACTAGCAATCC
|
61
|
63
|
291
|
AW098587 – 180
|
AGGAAGTTCGTGGTCGTGGG
|
TTGTGGCGTCCACATCGG
|
64
|
65
|
436
|
AW086962 – 158
|
TGGACTATTCCTTGGGCTTCTCG
|
CCCCGATTGCGCAGTATCC
|
65
|
65
|
287
|
AW086962 – 273
|
TCGAGCAAGGCAAGTCATAAGG
|
TCCGATGTTTCCTTCAGCGG
|
63
|
65
|
310
|
AW086962 – 333
|
TTCTCTACATGCTTGCCGC
|
TTTGATAGCACAGCAGGGTAATGC
|
60
|
64
|
272
|
AW098337 – 225
|
GAACCTGCACCACGACAAAGC
|
AAGCGTCGGCATTGCTCC
|
65
|
64
|
304
|
AW098364 – 121
|
TGGATATGGGTTGGCGGG
|
AGCTGCTTGATGTCGGCTCC
|
64
|
65
|
518
|
AW098799 – 185
|
TCAGGCTTTGCCTTGGTGG
|
AAGGTTGCTGGAGAAAGTAGCCC
|
64
|
64
|
354
|
AW098651 – 169
|
GCAATCCTGCTCTACCTTGCG
|
CGCTCCAACGGGTAAGGG
|
64
|
63
|
307
|
AW098361 – 258
|
AAATGCAGGCTGTGCGAGC
|
GCCATGACGACCTCCACG
|
64
|
63
|
834
|
AW086544 – 267
|
TTTCTTCGCAGAGCCCACG
|
GTGTTGCATCCTGGTCGTGC
|
64
|
65
|
331
|
AW086990 – 246
|
CCCAGACGGCATTCGAGG
|
TCATCCATTCGCATCTTCGC
|
65
|
64
|
499
|
AW086531 – 354
|
TTCAGCTGTCATGGCCTTCG
|
ACGCAAGCTTTGGCACCC
|
64
|
64
|
373
|
AW086934 – 65
|
TTTTTCAACGAAAATAGCGAGCC
|
CGAAGCTTTTGCAGGAACTCCC
|
63
|
66
|
265
|
AW086934 – 136
|
TCGAAAGGGACAAACGGGG
|
AAGGCTCTCTAATATGGGGGTCG
|
65
|
63
|
314
|
AW087013 – 133
|
CTTCGGAGCCACCATCCC
|
TTGTTGCCGAATGGGTCG
|
63
|
64
|
465
|
AW098823 – 115
|
AGCGAGCTTGCCTGCACC
|
GCTTGGCAAAGAGACCAGGC
|
65
|
64
|
393
|
AW098823 – 199
|
GCAACTTTGGTGAAGGCCG
|
CGGTCGCGGCAGTAGTAGC
|
63
|
64
|
308
|
AW098056 – 70
|
AAGAATGCAGTGTTTGGTGACAGC
|
CCACATCCGCCTTGAGAGC
|
64
|
64
|
542
|
AW097956 – 276
|
CCGCGACCAATGAGACCC
|
TGATCTGGATGCACGGGG
|
65
|
64
|
710
|
AW098620 – 189
|
GATGAACAACGCTCGCGG
|
TGGTGTCGGAGATGTGGGG
|
63
|
65
|
326
|
AW097987 – 87
|
GTCTTAACCTTGGATGGCCGC
|
GAGTGCTTTGAGTCATCGCTTCC
|
65
|
64
|
268
|
AW097987 – 167
|
TGTGGAGCCAACAGAGATTGAGG
|
TGCATGCCTGCAAATCAGC
|
65
|
64
|
328
|
AW097987 – 313
|
CATGCAGCCGTTAAGGAAAGG
|
TCAGCAATCCATTTTCAACCG
|
64
|
63
|
279
|
AW087075 – 240
|
TTGGTGATGCATACTCAAAGGTGG
|
GGTCATCGAGCTCTCCTGGC
|
65
|
64
|
452
|
AW087075 – 332
|
TCAACTGGCGATCAGAAGCC
|
CTGGCTGCACTAACACTGCC
|
63
|
61
|
232
|
AW098367 – 359
|
CAATTATCTCCAACGGCGGC
|
AGTGGTAATCAGCCGTCATCTCG
|
64
|
64
|
421
|
AW086525 – 450
|
ACTCCTGGTTGGTGGCCG
|
CTTCTTGAGGAAGTTGCAGAAGG
|
64
|
61
|
674
|
AW086525 – 514
|
TGATGGCAAGAAGGTGTCCG
|
ACCGCATGAAGTTGTGGGC
|
64
|
64
|
318
|
AW098078 – 239
|
CCTCAACACCAGGCTCAATGG
|
TGATCAAGGGCGCCAAGG
|
65
|
65
|
482
|
AW086765 – 100
|
TGACCCATGTGTTGATGATGAGG
|
ATTTCAATGCCAGATTCCAGC
|
65
|
61
|
484
|
AF309562 – 421
|
TCCCCTTCGAGGAAAACCC
|
CTGGCGACCAAAGCTCCG
|
63
|
65
|
425
|
AF309562 – 538
|
TGCTGGCGTTACAGACGACC
|
GAGCAGCAGCCCTCTGAGC
|
64
|
63
|
323
|
AW087021 – 183
|
GGTTGAAGCTGTGAAGTTGTTCG
|
GAGGACCTCTCTGGATGGGG
|
63
|
63
|
310
|
AW087021 – 312
|
AGCTTCTGCCATCCTCACTGC
|
AGTTGCGACGACGAGACGG
|
64
|
64
|
315
|
AW098247 – 82
|
ACGCGTCCATCTGCCAGG
|
AAGGTCGCCTCCACCTCG
|
65
|
63
|
461
|
AW086944 – 155
|
CTGCCATCCTTCCCGTCC
|
AAGCAGTTGGTGTTGGCGG
|
63
|
64
|
353
|
AW098672 – 254
|
TTCCTTGGGGGCCTCAGC
|
ACTGGCCTGGTCTCTTTGCC
|
65
|
63
|
313
|
AW087053 – 85
|
CGTCGTGAGCGTGAGGAGG
|
AGGTATCCAAGCTTCTCATTGTAGTCC
|
65
|
63
|
474
|
AW098317 – 317
|
TGATATGGGGTCTTCCAGGTCC
|
CGTTTTAATAAGAGGTCGACAGTGGC
|
64
|
65
|
498
|
AW086622 – 280
|
GCGAAGAGTGGGTAGCTCCG
|
AAGCCCGAGCCTGTGAGG
|
64
|
63
|
346
|
AW086841 – 85
|
TTCGGAAGCACAAAACTGACG
|
TCTCATCGCCGTTTAGCCG
|
63
|
64
|
279
|
AW098024 – 169
|
GGAAAGCTGTGACTGCACTTACCC
|
AATCTGGGCCTGGCCTTACC
|
65
|
64
|
578
|
AW086649 – 182
|
TGCATGAATCACAATGAAGCCC
|
ACGCATGCGCCATCTGC
|
65
|
65
|
463
|
AW098284 – 284
|
GCAGGACTGAGGAGTCGTCG
|
TTTTCCTAGTCCCGCACGC
|
63
|
63
|
309
|
AW098191 – 439
|
GACCCGCCACATGAATGC
|
CTTCCAGCTTGTGGACGGC
|
63
|
64
|
572
|
AW098191 – 543
|
TTGGAAAGGTTGTCAGCTTCTGC
|
TTCGAAGCATTTGGACCAGG
|
64
|
63
|
478
|
AW086824 – 186
|
GAACGAGGGCAAAACAACACG
|
TTGTTCGTATCATGAGTCCTTATTTGC
|
64
|
63
|
282
|
AW086824 – 253
|
GTGTACGTCATGGTGTCGATTGG
|
TGCTGGGTGGAGATGGTCC
|
64
|
64
|
456
|
AW086779 – 195
|
TGGTGCCAGTTTGAGGAAGC
|
AGCACCTCCGGCTTTGACC
|
63
|
64
|
431
|
AW098026 – 111
|
TGGCAGTGGAAAGCTGCG
|
CTCATTGGGCATGTGGATGG
|
64
|
64
|
395
|
AW086929 – 81
|
ATGCACACTGCATCCCTTTGC
|
AAATATGAAAGGAGGGGTCGC
|
65
|
61
|
407
|
AW086753 – 293
|
GGCAGCCCAATTTCATGAGG
|
ATTGCTTGGAGCCTCTCAATGG
|
64
|
64
|
458
|
AW086737 – 66
|
AACCTACTGGTCGACAAGAACTGG
|
CGAAGCACTTCCGGTGCC
|
62
|
65
|
464
|
AW098283 – 167
|
TGATGGATGCGCTTGTGG
|
TCTGCAAGAGAGCCTACCTTGACC
|
62
|
65
|
426
|
AW098019 – 120
|
ATCGGGATTAGGACCAAGGC
|
GATTCTGCCAGCGCATCC
|
62
|
62
|
271
|
AW098019 – 199
|
TGGAAAGGATGCGCTGGC
|
ATTGCGCATCATCCATACCG
|
65
|
63
|
265
|
AW098019 – 297
|
CTTGCGTGAGGACTATTTGGC
|
TTCTGCCCCTCAGAACCAGC
|
62
|
65
|
325
|
AW098233 – 116
|
TTATGCGCAGGAGCTTGGC
|
CCTCCTCCCGCTACCAACG
|
64
|
65
|
453
|
AW098233 – 233
|
GCAAATCCTGATGGCCGC
|
CCCGCATATTTGCCAATCC
|
65
|
63
|
281
|
AW086758 – 136
|
AAGGCTTTTGCACTGCACTCG
|
TGCTGCTCGCACTGGAGG
|
64
|
65
|
299
|
AW087065 – 177
|
CGGGAGCACTTAACGACGC
|
TCACACCTTCTGCTGTCTGG
|
64
|
60
|
575
|
AW098770 – 113
|
CCGTGAAGGACTGGGACAGG
|
TTGCACGCCTTGTATCCTCG
|
65
|
64
|
282
|
AW098258 – 180
|
TTTGCACCCATTGCCAACC
|
GCCGGGTTGTAAGCGAAGC
|
65
|
65
|
390
|
AW098797 – 116
|
AGGATCGGACTCCCTGCC
|
TTCCCAACTTGTCAACTGCCC
|
62
|
64
|
490
|
AW098643 – 123
|
GGAGGCTTTTGAGGCGAGG
|
CAATCAGCTGGCAATGAGCG
|
64
|
65
|
508
|
AW086790 – 163
|
TGGCAGAGTTTGATCGAGGC
|
TGAAGGTGCTCTGGAGCGG
|
63
|
65
|
591
|
AW098780 – 75
|
AGGCGTCAGTCTACGGAATTGG
|
TGCTCGCAAATCTTGCCC
|
64
|
63
|
379
|
AW098780 – 211
|
CCCACATCCCGATCCACG
|
ACAATTTTCGCTTCAACAAGATCTCC
|
65
|
64
|
324
|
AW086556 – 413
|
AAGGCAGACTCTCGGGCG
|
GCGTTCAGAAGGCCAATGC
|
64
|
64
|
458
|
AW098831 – 338
|
TTCAGTGGACGCGCTACCC
|
TCCACAGTTGAGTTCCGGTGC
|
64
|
65
|
456
|
AW086710 – 390
|
TTTCTCCGGGCTTCCATCC
|
AGGGCATTGCTCTCAGGGG
|
64
|
64
|
531
|
AW086768 – 261
|
CCTCCTCCCCATACGCTCC
|
CACAACAACAGCACAAAGCTGC
|
64
|
64
|
427
|
AW087072 – 205
|
CCGCGACCAATGAGACCC
|
TTGCGAGAAGTTGACCGTGG
|
65
|
64
|
387
|
AW087072 – 269
|
GCAGAAGGTCAAGAAGGCCC
|
CCGTTGAGAGTGAGGTCACGG
|
63
|
65
|
373
|
AW086870 – 57
|
AACCCCGCCTGCTTCACC
|
GGTGCATTTGGCTGTGCC
|
65
|
63
|
318
|
AW098197 – 197
|
ACGTGGATTTCAGGCGGC
|
CGTGCTTCTTCTTCAGTAGCAGGG
|
64
|
65
|
489
|
AW098074 – 139
|
CAGGGGAAAGGCACCTGG
|
CGGTTCCAGTGAGGATGCG
|
63
|
65
|
520
|
AW098521 – 456
|
ACTTCTCAGACGAGTTGGGGC
|
CGTGCCGAAAAGGTGCG
|
63
|
64
|
288
|
AW098301 – 160
|
GCTGTGCAGGCGTTGTGG
|
AGAAAGATGACGCAGATGGCG
|
65
|
64
|
599
|
AW087027 – 88
|
TGGGACTTCTACAAGCAAAAGTTGG
|
CAGCGCTTGATGCTTCG
|
64
|
60
|
268
|
AW087027 – 159
|
AGGTGCCAGAGTACAAGGATGACC
|
CTTCTGTTCCTGCACCCTCG
|
64
|
63
|
374
|
AW087027 – 246
|
GCTAAGGCCTCAGAGCAAGAGG
|
CTTCACTTGTGGGTGCTTTGC
|
63
|
63
|
291
|
AW086848 – 172
|
CAACCCTTTCATGCACGCC
|
CGCAACTCAGCCCTCACG
|
64
|
64
|
513
|
AW098187 – 139
|
AGTACAATCAGGCTGCCACGC
|
TTGAGTTGAACTTCATTTACCTGCC
|
64
|
62
|
306
|
AW086969 – 51
|
TCGCATTTATGGCAGAGCAGG
|
AGATTCTTCAGACGAACAGCCG
|
65
|
63
|
280
|
AW086692 – 252
|
CCGAATCATCAGATGCCAGG
|
GTCTCGCAGCCGAGTTGG
|
63
|
63
|
420
|
BI894286 – 170
|
TGGACGAGCTGAGCGAGG
|
TTACGCCATGTCCTTCGCC
|
63
|
64
|
494
|
AW086973 – 252
|
GGATGATTCCGCCAAACAGG
|
TCTACGACGGCATCAGGGC
|
64
|
64
|
309
|
AW098153 – 431
|
CGAAGACGGCCTTGCACC
|
CAAATTGGAGCAGCTGTGGC
|
65
|
64
|
448
|
AW098812 – 178
|
ACTGGCCCAGCCTTTCCG
|
AGAAGTAGCCCCACTGCATCG
|
65
|
63
|
430
|
AW098812 – 326
|
TGAGATTGGGTTGTTCGATGG
|
CCGCCGTCCACAATCTCG
|
63
|
66
|
457
|
AW086545 – 249
|
TTCCTTGGGGGCCTCAGC
|
GCCGGTGTGCAAATTGAGG
|
65
|
64
|
330
|
AW086575 – 297
|
CATCTAGGTATTGTCGAGTCCCG
|
TGAAAGTGGCAAGATGACCAAGG
|
62
|
65
|
459
|
AW086575 – 420
|
AGCAACTGCATCAATAAATTCCTCG
|
TGGACCCGTGGTCTTAGCC
|
64
|
63
|
317
|
AW086781 – 77
|
CTCGCGTTGCTGGTGTCG
|
TTCTGCGCATCTTCTTTTTGC
|
65
|
62
|
421
|
AW087048 – 137
|
GAAGGCTGCAATTCAGGAAACG
|
TGAAAGAGGTTTCTTTGGTTTGTTGG
|
65
|
65
|
257
|
AW087048 – 215
|
GAGAAGAAGAAGGCCAGGGACC
|
GTGGGCATTCGTTTCCGC
|
64
|
64
|
515
|
AW098758 – 58
|
TATTTGCTCCAGGATGCTGATGG
|
GGCTTCATCGTCAGTCACGC
|
65
|
64
|
319
|
AW086711 – 314
|
TGAATCCGGCTGTCAAATCG
|
GCGAGCTGCTGGTTCTGAGG
|
64
|
65
|
300
|
AW086711 – 405
|
TAGATGAGGTCACTCAAACGCTGC
|
AAATCGGTATGGATGATGCG
|
64
|
60
|
438
|
AW086711 – 484
|
TTGCGCATCATCCATACCG
|
AGTTGCAAAACTCTTGCGTGAGG
|
63
|
64
|
305
|
AW098776 – 59
|
GTCAGGCTCTGGCTGACG
|
GCCAGCCTTTGCTTGATGTCC
|
60
|
66
|
314
|
AW098776 – 152
|
GCCAAGACGAACATGAATGTGG
|
GCAGGAACCTGATGCTGGC
|
64
|
64
|
359
|
AW098585 – 138
|
CCTCGCCTCATGTCCTGC
|
AGCACGTGCGTAGTTCCCG
|
63
|
64
|
335
|
AW098746 – 319
|
GGTTTCATCTCTGGCGCTTCG
|
CCCATGAGGTCAAAGATGAGGG
|
66
|
65
|
249
|
AW098746 – 414
|
CCACCATGGTCCACTTCATAGC
|
CTGAAGCGATCCCCCACC
|
63
|
63
|
421
|
AW098409 – 116
|
CAGAGATGGTCGTGGGTTGG
|
CCCAACATCATCGTCTGAGGG
|
63
|
64
|
447
|
|
|
|
|
|
|
Primer less than 25 nucleotides from intron
|
|
|
|
|
AW086551 – 330
|
TTCTGTTTCCAACAGGCCG
|
GTGCGCAAATTCACAGAGCG
|
62
|
65
|
307
|
AW086551 – 425
|
TCTCGCTCTGTGAATTTGCG
|
CGATGTCAGAAGGCAGGTGG
|
62
|
64
|
231
|
AW086636 – 421
|
TCAGCTGACTTCGCGTTTGC
|
GTTGGCTCAAGGAAAGGAGC
|
65
|
61
|
314
|
AW086700 – 323
|
AGTCAAGCGGGCCCTTCC
|
ATGTGAAGTGCCAGGGCTGC
|
64
|
65
|
301
|
AW086752 – 361
|
AACCAGAGCCCCAACCCG
|
ACAATCAGCGTGACCTCAAACG
|
65
|
64
|
395
|
AW086783 – 208
|
CGTCTCATCATGCGCAACG
|
CTTCGCGCTCAGACTCAAGG
|
65
|
63
|
665
|
AW086798 – 387
|
GCATGGAACTCCCGGAACC
|
CATCAAACTCCCACAACTCATTTCC
|
65
|
64
|
393
|
AW086855 – 57
|
GTGATCATGGCGGGGAGG
|
TCCTCCGTCCTACAGTCGCC
|
64
|
64
|
263
|
AW086855 – 188
|
CCTGTTGTCGCAGCCAGC
|
CGTCGTCAACGATTGTAGGG
|
64
|
61
|
321
|
AW086856 – 213
|
TCACGGATCTTTGGCCCG
|
TTGGGGCCCCTCTTCTTACC
|
65
|
64
|
276
|
AW087036 – 57
|
AGAAGGAGTCTACTCTGCATCTGG
|
CCTCAAGCTGCTTTCCAGCG
|
60
|
65
|
425
|
AW087051 – 351
|
TTCACAACAGGCAGACCCG
|
GACAGTGGCTCTCTGGAAGGC
|
63
|
63
|
328
|
AW087074 – 125
|
CAGGCTGGTCCGCTTTGG
|
CCGTTAACCTGAATATCCTTCACACC
|
65
|
64
|
395
|
AW087074 – 171
|
TGTGAAGGATATTCAGGTTAACGGC
|
TGATGGTTGAGCTCCGACG
|
64
|
63
|
237
|
AW097915 – 117
|
GGGAGCAACCTGACCCTTCC
|
CGCTCGAGATCGTAGCCTTGC
|
65
|
66
|
252
|
AW097968 – 35
|
AGTGCGGTGCCAACTGCG
|
CCGCACTTGCCGTTCTCC
|
67
|
65
|
418
|
AW097979 – 42
|
GATGGCTCCAGCCCTTCG
|
AGCTCTCCCCTGATGTTTTCAGC
|
64
|
65
|
318
|
AW097979 – 92
|
AACTGGCTGAAAACATCAGGGG
|
AGTGACGCTGGCAACTCCC
|
64
|
63
|
361
|
AW098011 – 47
|
TGTTGCAGCTTGTGTACTTTCTGTACC
|
ATGGGTGCGCCTGAAATCG
|
64
|
66
|
303
|
AW098051 – 84
|
GATTCCGTCACTGTCGGTGG
|
GTGCCGAAACTGGTGCCC
|
63
|
65
|
519
|
AW098200 – 145
|
CAATGACACCAGGCTCCAACC
|
TGCACCTTGTGGGGCAGC
|
65
|
66
|
275
|
AW098203 – 61
|
CAAGTTCTTGCAGGAAACATTTGG
|
CCATGCTTGCTGACCGGG
|
64
|
66
|
464
|
AW098216 – 110
|
AGGGTGTCAATACGTCCAAGG
|
TGGCACCGAGAGACGAAGG
|
61
|
64
|
460
|
AW098262 – 128
|
ACTACGTTGCCCACGACGC
|
TTCGTAAACATCCTTGCCAGC
|
64
|
62
|
233
|
AW098262 – 171
|
GGATGCAACTGGTGCTGGC
|
GATCAGATCCGAAGTCGAAACTCC
|
65
|
64
|
224
|
AW098263 – 244
|
TAGCATGGAGCACTGATCGGG
|
CAATCCAACGTCCAAAGTAAACTGC
|
65
|
64
|
281
|
AW098272 – 223
|
CATTTGTGTGTTCCTTCTGCCG
|
TGAGTCTCTTGAAGTCTCTTCATTTCC
|
64
|
62
|
190
|
AW098291 – 131
|
TGTAGGCCGAATAGCACTTGG
|
TGGACCAGTAGTCATGTTGAATGC
|
62
|
63
|
245
|
AW098300 – 108
|
TGCAACGGTGTCGTTGTGC
|
ACCCGTGAATCTTGATGAGGTCC
|
65
|
65
|
453
|
AW098306 – 52
|
GTTGCGCAGGGTTGAGCC
|
TGGCCTCTCTGTTGCCAGC
|
65
|
65
|
450
|
AW098419 – 60
|
CGCCAGATTCAGGAGCAACC
|
CTGGGGTACATGAACACCCTTACG
|
65
|
65
|
223
|
AW098448 – 78
|
ATGTCAAGTGCCAAGGATGC
|
CACAGCACCGTGGAGCAGC
|
61
|
66
|
276
|
AW098461 – 262
|
CACACTCCTTTCCAATGGGGC
|
ACAATCGGGAGTCATTGTGACC
|
66
|
63
|
269
|
AW098461 – 303
|
GGGGTCACAATGACTCCCG
|
CAAGCCCTGAGGCGCAAAGC
|
63
|
69
|
330
|
AW098472 – 354
|
GTAGGATACGGTTTGAGGGCTGC
|
GCTCTTGAAGAAGAAGTTCGGG
|
65
|
61
|
493
|
AW098535 – 258
|
TCGAACAAGCTGAAGCCC
|
GAAGTTCGCGTCTGTGCCC
|
60
|
64
|
431
|
AW098580 – 204
|
CAAGACCCCACCATCTACAGGC
|
CCCTTCACCTTCTCCACAGAGC
|
65
|
64
|
315
|
AW098597 – 63
|
GGAGCTGGTGACAGTGTGAAGG
|
AGCAGCCATCAGACCCCC
|
64
|
63
|
273
|
AW098717 – 46
|
TCACGGCTCAAACTCTGATTAAATAGC
|
AAATCCAAGGCCAGAACCCC
|
64
|
64
|
334
|
|
|
|
|
|
|
*Size indicates the predicted PCR product size on the Physcomitrella patens genome sequence
|
|
|